《植物生理学报》 2012, 48(3): 251-259

不同光质对烟草叶片组织结构及Rubisco羧化酶活性和rbc、rca基因表达

柯学¹, 李军营^2,*, 徐超华¹, 龚明^1,**

1云南师范大学生命科学学院, 生物能源持续开发利用教育部工程研究中心, 云南省生物质能与环境生物技术重点实验室,

通信作者：李军营；E-mail: gongming63@163.com；Tel: 0871-5941370

摘　要：

通过对烟草植株覆盖白、红、黄、蓝、紫色滤膜获得不同光质, 研究了烟草叶片在7~70 d的生长发育期内, 不同光质处理对烟叶组织结构特征、核酮糖1,5-二磷酸羧化酶/加氧酶(Rubisco)羧化酶活性、Rubisco基因(rbc)表达及其活化酶(Rca)基因(rca)表达的影响。结果表明, 与黄膜处理下生长的烟叶相比, 红、蓝、紫膜处理下生长的烟叶有较高的叶片厚度、栅栏组织厚度、海绵组织厚度、栅栏细胞密度和较小的组织空隙率。此外, 红、蓝、紫膜处理的叶片有较高的Rubisco羧化酶活性和净光合速率及较强的rbc和rca基因表达。实验结果表明不同光质对烟草叶片的组织结构特征有显著影响, 光质可能通过影响Rubisco羧化酶活性进而影响叶片光合效率, 而光质、叶片组织结构和光合效率之间存在某种程度的相互联系。

关键词：光质; 烟草叶片; 解剖结构; 核酮糖1,5-二磷酸羧化酶/加氧酶

收稿：2011-11-03   修定：2012-01-31

资助：国家自然科学基金(30460016)和中国烟草总公司云南省公司科技计划项目(2011YN03和2010YN03)。

Effects of Different Light Quality on Anatomical Structure, Carboxylase Activity of Ribulose 1,5-Biphosphate Carboxylase/Oxygenase and Expression of rbc and rca Genes in Tobacco (Nicotiana tabacum L.) Leaves

KE Xue¹, LI Jun-Ying^2,*, XU Chao-Hua¹, GONG Ming^1,**

¹School of Life Sciences, Yunnan Normal University, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Kunming 650500, China; ²Yunnan Academy of Tobacco Agricultural Sciences, Yuxi, Yunnan 653100, China

Corresponding author: LI Jun-Ying; E-mail: gongming63@163.com; Tel: 0871-5941370

Abstract:

By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the effects of light quality on anatomical structure, carboxylase activity of ribulose 1,5-biphosphate carboxylase/oxygenase (Rubisco), and expression of rbc and rca genes were investigated in tobacco leaves aged from 7 to 70 days. Compared to the leaves grown under yellow film, red, blue and purple film treatments increased the leaf thickness, palisade and spongy parenchyma thickness, and density of palisade cells, but lowered the tissue interspace in the tobacco leaves. In addition, the leaves with red, blue and purple film treatments showed higher carboxylase activity of Rubisco and net photosynthetic rate and stronger expression of rbc and rca genes than those with yellow film treatment. These results indicated that, different light quality obviously influenced the anatomical structure, carboxylase activity of Rubisco and further photosynthesis efficiency of tobacco leaves, and there was a possible relationship among light quality, anatomical structure and photosynthesis efficiency of tobacco leaves to some extent.

Key words: light quality; tobacco leaves; anatomical structure; ribulose 1,5-biphosphate carboxylase/oxygenase